The OAV B-ZOOM is an On-Axis Video-microscope with a hybrid zoom system used for parallax free observation of micrometer sized crystals.
It is optimized for MX crystallography beamlines and high throughput stations in Synchrotrons.
The OAV B-ZOOM is the next evolution of the OAV Ultra-Zoom: it combines the EMBL patented Objective Head of the OAV with a new zooming and visualization module, allowing instant zoom change and integrating a multi-camera video server.
Optimal working distance
Instant zoom change
- Uses two video cameras
- Optimized for high throughput screening
Integrated video server
- virtual single-camera operation
- Bandwidth 200 MP/s with RGB color treatment
- Flexible camera choice
As its predecessor, the OAV B-Zoom is equipped with the objective lens system developed by the EMBL-Grenoble instrumentation group. The lens system has a central hole, coaxial with the optical axis, by which the X-ray beam passes through.
This way, the OAV B-Zoom provides an on-axis visualization allowing in situ perfect alignment of the sample.
The hardware offers x5 and x30 optical magnifications, a numerical aperture of 0.28 and a working distance of 34.8 mm (31.8 mm if a protective glass is added).
Operated with powerful video cameras, the OAV B-ZOOM provides excellent optical performance, with a resolution of 0.16 µm/px @zoom max and 1.9 µm/px @ zoom min. Consequently, the OAV allows distinguishing objects as small as 1 µm or even smaller under optimal conditions.
Instant Zoom Change
The OAV B-Zoom associates two video cameras, one for a « low zoom » level, and the second for a « high zoom » level. This Hybrid Zoom Setup uses images from both cameras to emulate a single camera equipped with a zooming device. With this setup you can switch between any zoom level instantaneously.
This configuration is especially well suited for highly automated end-stations. On modern high-throughput beamlines, zooming frequencies of 200 times per day or even more are common. Without motorized mechanics, the OAV B-Zoom is far more reliable than other systems and its instant zoom allows for faster operation.
The zoom level range of the virtual hybrid camera (with 1280 x 1048px) is x2.5 to x30, giving a x12 range. It is possible to go beyond 100% by using up-scaling (interpolation).
Compared to the previous motorized OAV system, the OAV B-ZOOM presents wider range and field. In addition, the OAV B-Zoom provides a series of pre-configuration zoom levels between x2.5 – x12 and between x20 – x30, for which average sharpness is better.
Integrated Video Server
A Video Server designed by Arinax is delivered with the OAV B-ZOOM. The purposes of the server are to:
Accelerate the integration of the cameras into the customer applications
which avoid development for specific camera vendor interface. Currently, the server exports several control interfaces: DLL API, Tango, EPICS, Mjpeg web server (see server architecture on the figure)
Remove the CPU load burden
of resizing images out of the display application
Ensure maximum frame rate
by an optimized program written in C++ and using state of the art image processing
Simulate the zoom control
by choosing the right camera and resizing images
The OAV B-ZOOM is integrated into the MD3-UP diffractometer. Within the diffractometer software, a “three click-centering” function gives the possibility in only three clicks to:
Localize the X-ray beam
Align the sample with the X-ray beam position
Center the sample with respect to the X-ray beam
|Design resolution @max zoom||0.16 µm/px|
|Design resolution @min zoom||1.9 µm/px|
|Hybrid zoom range (combined image)||2.5 to 30|
|Field of view||2.4 x 1.9 mm @ Zoom min;
0.38 x 0.24 mm @ zoom max
|High resolution drilled objective lens||Coaxial hole: 1 mm diameter|
|– Working distance||31.8 or 34.8 mm|
|– Magnification||x5 to x30|
|– Numerical aperture||0.28|
|Spatial resolution||CCD: 2560×2048 px|
|Power supply||POE 12 V (Power Over Ethernet)|
|Output image size||1280×1024 px recommended. Configurable.|
|Image modes||Simultaneous Raw Images, Auto-selection, Combined|
|Image formats||Mono, RGB, BGR|
|Compatible cameras||Genicam, Ueye, Jai, Basler,Prosilica, Mako|
|CPU requirements||22 FPS delivered by the Video Server tested on a PC with one i7-6700 CPU: workload = 25% for two cameras processed in parallel, server output ROI 1280×1024, Bayer conversion included|
|Width x Height x Depth||94 x 362 x 88.2 mm|